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primescripttm ii 1st strand cdna synthesis kit  (TaKaRa)


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    TaKaRa primescripttm ii 1st strand cdna synthesis kit
    Primescripttm Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+ii+1st+strand+cdna+synthesis+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13123502-115-29-36
    Average 99 stars, based on 11018 article reviews
    primescripttm ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Identification and characterization of WpDestabilase, a novel destabilase from the salivary glands of Whitmania pigra.
    Article Snippet: Whitmania pigra is one of the primary sources of leeches used in traditional Chinese medicine, renowned for its anticoagulant and thrombolytic properties.. In this study, a salivary gland transcriptome database of W. pigra was constructed, and 7 anticoagulation-related transcripts were identified, one of which was designated as “WpDestabilase”.. The unigenes producing these transcripts were functionally annotated to proteins with potential pharmacological activities, including fibrinolytic enzymes, neutrophil elastase inhibitors, elastase inhibitors, and factor Xa inhibitors.

    Article Title: Nucleic acid molecules and dual-functional peptides having antiviral activity and delivery activity, compositions and methods thereof
    Article Snippet: .. RNA was reverse transcribed to cDNA using primer Uni-12 and PrimeScriptTM II 1st Strand cDNA synthesis Kit (Takara, Cat #6210A) using GeneAmp® PCR system 9700 (Applied Biosystems, USA). ..

    Article Title: Solanaceous plant and solanaceous plant cell having resistance to tomato spotted wilt virus, and method for producing solanaceous plant
    Article Snippet: .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation). .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation).

    cDNA Synthesis:

    Article Title: Identification and characterization of WpDestabilase, a novel destabilase from the salivary glands of Whitmania pigra.
    Article Snippet: Whitmania pigra is one of the primary sources of leeches used in traditional Chinese medicine, renowned for its anticoagulant and thrombolytic properties.. In this study, a salivary gland transcriptome database of W. pigra was constructed, and 7 anticoagulation-related transcripts were identified, one of which was designated as “WpDestabilase”.. The unigenes producing these transcripts were functionally annotated to proteins with potential pharmacological activities, including fibrinolytic enzymes, neutrophil elastase inhibitors, elastase inhibitors, and factor Xa inhibitors.

    Article Title: Nucleic acid molecules and dual-functional peptides having antiviral activity and delivery activity, compositions and methods thereof
    Article Snippet: .. RNA was reverse transcribed to cDNA using primer Uni-12 and PrimeScriptTM II 1st Strand cDNA synthesis Kit (Takara, Cat #6210A) using GeneAmp® PCR system 9700 (Applied Biosystems, USA). ..

    Article Title: Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
    Article Snippet: The GFP and DAPI fluorescence signals were separately visualized under a laser scanning confocal microscope (Zeiss LSM900, Germany). .. Total RNA was extracted from leaf, stem, and flower tissues of P. polyphylla using TaKaRa MiniBEST Plant RNA Extraction Kit (Takara, China), and the first-strand cDNA was synthesized through PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, China). .. For the PpUGTs expression analysis, qRT-PCR was performed on a qTOWER 3 G Real-Time PCR Thermal Cycler (Analytik Jena AG, Germany) using the PerfectStart® Green qPCR SuperMix (TransGen Biotech, China) according to the product manual.

    Article Title: The SUMO specific peptidase SENP3 promotes papillary thyroid cancer progression by deSUMOylation of SETDB1 and regulating GLI2 transcription.
    Article Snippet: .. PrimeScriptTM II 1st Strand cDNA Synthesis kit from Takara (Dalian, China) was used for gene expression detection. .. Real-time PCR analyses were conducted with the SYBR Premix Ex Taq II (Takara, Dalian, China) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) under the following conditions: 95 °C for 1 min, and then 95°C for 20 s, 56°C for 10 s and 72°C for 15 s for 35 cycles.

    Article Title: Solanaceous plant and solanaceous plant cell having resistance to tomato spotted wilt virus, and method for producing solanaceous plant
    Article Snippet: .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation). .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation).

    Article Title: Exploring the immunological functions of calreticulin 3a from Scomber japonicus (SjCalr3a): insight into antimicrobial defense, cytoprotection, and pro-inflammatory responses.
    Article Snippet: Calreticulin 3a (Calr3a) is a ubiquitously expressed calcium (Ca2+)-binding chaperone protein involved in various cellular processes, including intracellular calcium homeostasis; antimicrobial defense; regulation of cell proliferation and migration; and modulation of immune-related gene expression.. Although its role in mammalian immunity is well-established, its functions in teleosts remain poorly understood.. In the present study, we identified and functionally characterized the Calr3a homolog from chub mackerel (Scomber japonicus), termed SjCalr3a.

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy
    Article Snippet: Absorbance was determined using a SpectraMax i3x plate reader (293817527; Molecular Devices, USA) at the wavelength of 450 nm (correction 540 nm). .. Total RNA was extracted from HCAECs using TRIzol reagent (15596026CN; Invitrogen, USA). cDNA was synthesized using a PrimeScriptTM II 1st Strand cDNA Synthesis Kit (6210A; Takara, Japan). .. PCR was performed using PrimeScriptTM II High Fidelity RT-PCR Kit (R023A; Takara, Japan).

    Article Title: Analysis of Axillary Bud Germination Regulatory Network in Sugarcane Based on Transcriptome and Weighted Gene Co-Expression Network Analysis.
    Article Snippet: .. Total RNA was extracted using the TRIzol® Kit (Coolaber, Beijing, China). mRNA was enriched using oligo(dT) magnetic beads, fragmented, and converted into cDNA with the PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TaKaRa, Dalian, China) to construct the library. .. Double-end sequencing was performed on the Illumina HiSeqTM 2000 platform (Illumina, Inc., San Diego, CA, USA) with three biological replicates per sample.

    Cloning:

    Article Title: Identification and characterization of WpDestabilase, a novel destabilase from the salivary glands of Whitmania pigra.
    Article Snippet: Whitmania pigra is one of the primary sources of leeches used in traditional Chinese medicine, renowned for its anticoagulant and thrombolytic properties.. In this study, a salivary gland transcriptome database of W. pigra was constructed, and 7 anticoagulation-related transcripts were identified, one of which was designated as “WpDestabilase”.. The unigenes producing these transcripts were functionally annotated to proteins with potential pharmacological activities, including fibrinolytic enzymes, neutrophil elastase inhibitors, elastase inhibitors, and factor Xa inhibitors.

    Polymerase Chain Reaction:

    Article Title: Nucleic acid molecules and dual-functional peptides having antiviral activity and delivery activity, compositions and methods thereof
    Article Snippet: .. RNA was reverse transcribed to cDNA using primer Uni-12 and PrimeScriptTM II 1st Strand cDNA synthesis Kit (Takara, Cat #6210A) using GeneAmp® PCR system 9700 (Applied Biosystems, USA). ..

    Article Title: Solanaceous plant and solanaceous plant cell having resistance to tomato spotted wilt virus, and method for producing solanaceous plant
    Article Snippet: .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation). .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation).

    RNA Extraction:

    Article Title: Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
    Article Snippet: The GFP and DAPI fluorescence signals were separately visualized under a laser scanning confocal microscope (Zeiss LSM900, Germany). .. Total RNA was extracted from leaf, stem, and flower tissues of P. polyphylla using TaKaRa MiniBEST Plant RNA Extraction Kit (Takara, China), and the first-strand cDNA was synthesized through PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, China). .. For the PpUGTs expression analysis, qRT-PCR was performed on a qTOWER 3 G Real-Time PCR Thermal Cycler (Analytik Jena AG, Germany) using the PerfectStart® Green qPCR SuperMix (TransGen Biotech, China) according to the product manual.

    Synthesized:

    Article Title: Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
    Article Snippet: The GFP and DAPI fluorescence signals were separately visualized under a laser scanning confocal microscope (Zeiss LSM900, Germany). .. Total RNA was extracted from leaf, stem, and flower tissues of P. polyphylla using TaKaRa MiniBEST Plant RNA Extraction Kit (Takara, China), and the first-strand cDNA was synthesized through PrimeScriptTM II 1st Strand cDNA Synthesis Kit (Takara, China). .. For the PpUGTs expression analysis, qRT-PCR was performed on a qTOWER 3 G Real-Time PCR Thermal Cycler (Analytik Jena AG, Germany) using the PerfectStart® Green qPCR SuperMix (TransGen Biotech, China) according to the product manual.

    Article Title: Solanaceous plant and solanaceous plant cell having resistance to tomato spotted wilt virus, and method for producing solanaceous plant
    Article Snippet: .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation). .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation).

    Article Title: Exploring the immunological functions of calreticulin 3a from Scomber japonicus (SjCalr3a): insight into antimicrobial defense, cytoprotection, and pro-inflammatory responses.
    Article Snippet: Calreticulin 3a (Calr3a) is a ubiquitously expressed calcium (Ca2+)-binding chaperone protein involved in various cellular processes, including intracellular calcium homeostasis; antimicrobial defense; regulation of cell proliferation and migration; and modulation of immune-related gene expression.. Although its role in mammalian immunity is well-established, its functions in teleosts remain poorly understood.. In the present study, we identified and functionally characterized the Calr3a homolog from chub mackerel (Scomber japonicus), termed SjCalr3a.

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy
    Article Snippet: Absorbance was determined using a SpectraMax i3x plate reader (293817527; Molecular Devices, USA) at the wavelength of 450 nm (correction 540 nm). .. Total RNA was extracted from HCAECs using TRIzol reagent (15596026CN; Invitrogen, USA). cDNA was synthesized using a PrimeScriptTM II 1st Strand cDNA Synthesis Kit (6210A; Takara, Japan). .. PCR was performed using PrimeScriptTM II High Fidelity RT-PCR Kit (R023A; Takara, Japan).

    Gene Expression:

    Article Title: The SUMO specific peptidase SENP3 promotes papillary thyroid cancer progression by deSUMOylation of SETDB1 and regulating GLI2 transcription.
    Article Snippet: .. PrimeScriptTM II 1st Strand cDNA Synthesis kit from Takara (Dalian, China) was used for gene expression detection. .. Real-time PCR analyses were conducted with the SYBR Premix Ex Taq II (Takara, Dalian, China) on an ABI 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA) under the following conditions: 95 °C for 1 min, and then 95°C for 20 s, 56°C for 10 s and 72°C for 15 s for 35 cycles.

    Amplification:

    Article Title: Solanaceous plant and solanaceous plant cell having resistance to tomato spotted wilt virus, and method for producing solanaceous plant
    Article Snippet: .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation). .. Using the total RNA as a template, cDNA was synthesized by reverse transcription using PrimescriptTM II 1ST Strand cDNA synthesis kit (manufactured and sold by TAKARA BIO® INC.), and amplification products were obtained by PCR (Conditions: 2 minutes at 95° C.; 31 cycles of 30 seconds at 94° C., 40 seconds at 56° C., and 45 seconds at 72° C.; and 3 minutes at 72° C.) by using Tospovirus detection primer 3 (5′-CTGTARTGKTCCATWGCARCA (SEQ ID NO:6)) and primer 4 (5′-GAYATGACYTTCMGAAGRCTTGAT (SEQ ID NO:7)) and GO Taq® Green master polymerase (manufactured and sold by Promega® Corporation).

    Concentration Assay:

    Article Title: Exploring the immunological functions of calreticulin 3a from Scomber japonicus (SjCalr3a): insight into antimicrobial defense, cytoprotection, and pro-inflammatory responses.
    Article Snippet: Calreticulin 3a (Calr3a) is a ubiquitously expressed calcium (Ca2+)-binding chaperone protein involved in various cellular processes, including intracellular calcium homeostasis; antimicrobial defense; regulation of cell proliferation and migration; and modulation of immune-related gene expression.. Although its role in mammalian immunity is well-established, its functions in teleosts remain poorly understood.. In the present study, we identified and functionally characterized the Calr3a homolog from chub mackerel (Scomber japonicus), termed SjCalr3a.

    Magnetic Beads:

    Article Title: Analysis of Axillary Bud Germination Regulatory Network in Sugarcane Based on Transcriptome and Weighted Gene Co-Expression Network Analysis.
    Article Snippet: .. Total RNA was extracted using the TRIzol® Kit (Coolaber, Beijing, China). mRNA was enriched using oligo(dT) magnetic beads, fragmented, and converted into cDNA with the PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TaKaRa, Dalian, China) to construct the library. .. Double-end sequencing was performed on the Illumina HiSeqTM 2000 platform (Illumina, Inc., San Diego, CA, USA) with three biological replicates per sample.

    Construct:

    Article Title: Analysis of Axillary Bud Germination Regulatory Network in Sugarcane Based on Transcriptome and Weighted Gene Co-Expression Network Analysis.
    Article Snippet: .. Total RNA was extracted using the TRIzol® Kit (Coolaber, Beijing, China). mRNA was enriched using oligo(dT) magnetic beads, fragmented, and converted into cDNA with the PrimeScriptTM II 1st Strand cDNA Synthesis Kit (TaKaRa, Dalian, China) to construct the library. .. Double-end sequencing was performed on the Illumina HiSeqTM 2000 platform (Illumina, Inc., San Diego, CA, USA) with three biological replicates per sample.



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